Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3

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Quantitation of membrane glycoprotein IIIa on intact human platelets using the monoclonal antibody, AP-3.

A murine monoclonal antibody specific for glycoprotein (GP)IIIa was prepared by immunization with a GPIIb- and GPIIIa-enriched Triton X-114 extract of platelet membranes. This antibody, designated AP-3, was shown by indirect immunoprecipitation to react solely with GPIIIa derived from either P1A1-positive or -negative individuals. The epitope on GPIIIa recognized by AP-3 is expressed on dissoci...

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Quantitation of Membrane Glycoprotein lila on Intact Human Platelets Using the Monoclonal Antibody, AP-3

A murine monoclonal antibody specific for glycoprotein (GP)llla was prepared by immunization with a GPIIband GPIIla-enriched Triton X-1 14 extract of platelet membranes. This antibody, designated AP-3, was shown by indirect immunoprecipitation to react solely with GPIIIa derived from either P1 Alpositive or -negative individuals. The epitope on GPIIIa recognized by AP-3 is expressed on dissocia...

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Interaction of AP-2, a monoclonal antibody specific for the human platelet glycoprotein IIb-IIIa complex, with intact platelets.

A murine monoclonal antibody, designated AP-2, reacts specifically with the complex formed by human platelet membrane glycoproteins IIb and IIIa, but does not react at all with the individual glycoproteins. Purified AP-2 covalently coupled to Sepharose CL4B was used as an immunoadsorbent column to purify the IIb-IIIa complex from a preparation of Triton X-100-solubilized human platelet proteins...

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Glycoprotein IIIa is phosphorylated in intact human platelets.

The glycoprotein IIb-IIIa complex (GP IIb-IIIa) is a multifunctional transmembrane protein on platelets. Its most completely described function is as a fibrinogen receptor that mediates platelet aggregation, but it is also involved in clot retraction, signal transduction, calcium transport, and other events. However, the mechanisms that regulate the functions of GP IIb-IIIa during platelet acti...

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A human monoclonal autoantibody recognizes a neoantigen on glycoprotein IIIa expressed on stored and activated platelets.

We prepared a heterohybrid cell line that secretes a human IgM monoclonal autoantibody that recognizes an antigen found on thrombin-activated or stored platelets. The surface expression of the epitope recognized by this autoantibody, 5E5, increases with time as platelets age in vitro, suggesting that it may represent a senescence or activation-specific antigen. 5E5 binds to the purified platele...

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ژورنال

عنوان ژورنال: Blood

سال: 1985

ISSN: 0006-4971,1528-0020

DOI: 10.1182/blood.v65.1.227.bloodjournal651227